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Volume 74, Issue 4

sp. nov. and sp. nov., isolated from grassland soil and showing plant growth promoting potential No Access

Abstract

Two Gram-negative bacterial strains designated MMS20-SJTN17 and MMS20-SJTR3 were isolated from a grassland soil sample, and taxonomically characterized using a polyphasic approach. The 16S rRNA gene sequence analysis indicates that both strains belong to the genus of the class , with strain MMS20-SJTN17 being mostly related to WSM5005 (96.45 % sequence similarity) and strain MMS20-SJTR3 to STM678 (98.59 % sequence similarity). MMS20-SJTN17 could grow at 15–40 °C (optimum, 25–30 °C) and at pH 6.0–8.0 (optimum, pH 6.0–7.0), whereas MMS20-SJTR3 could grow at 10–40 °C (optimum, 30–37 °C) and at pH 6.0–8.0 (optimum, pH 6.0). Both strains tolerated up to 1 % (w/v) NaCl (optimum, 0 %). The major fatty acids of MMS20-SJTN17 were C and C cyclo ω8, and those of MMS20-SJTR3 were C cyclo and a summed feature comprising C ω7 and/or C ω6. The major isoprenoid quinone of both strains was ubiquinone-8 and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Regarding plant growth promoting potential, both strains were capable of producing indole acetic acid, siderophore and 1-aminocyclopropane-1-carboxylic acid deaminase, and also showed phosphate-solubilizing activity. A genome-based comparison using orthologous average nucleotide identity and digital DNA–DNA hybridization values indicates that strain MMS20-SJTN17 shares highest relatedness with JC2948 and MMS20-SJTR3 with G-4-1-8, with values clearly below the cutoffs for species distinction. Examination of biosynthetic gene clusters responsible for secondary metabolite production reveals unique characteristics distinguishing each strain from closely related species. On the basis of genotypic, phenotypic, chemotaxonomic and phylogenomic data, each strain should be classified as a novel species of the genus , for which the names sp. nov. (=MMS20-SJTN17=LMG 32366=KCTC 82783) and sp. nov. (=MMS20-SJTR3=LMG 32367=KCTC 82784) are proposed.

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  • Accepted:
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Keyword(s): Paraburkholderia sejongensis , Paraburkholderia translucens , park soil and plant growth promoting potential
Funding
This study was supported by the:
  • National Institute of Biological Resources
    • Principle Award Recipient: SeungBum Kim
© 2024 The Authors
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Paraburkholderia translucens sp. nov. and Paraburkholderia sejongensis sp. nov., isolated from grassland soil and showing plant growth promoting potential
Int J Syst Evol Microbiol 74, 006347 (2024); https://doi.org/10.1099/ijsem.0.006347
/content/journal/ijsem/10.1099/ijsem.0.006347
/content/journal/ijsem/10.1099/ijsem.0.006347
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